Cylinder-shaped structures measure saturated and unsaturated fatty acids
FRANKFURT.Not only humans but also each of their body cells must watch their fat balance. Fats perform highly specialised functions, especially in the cell membrane. A research group at the Buchmann Institute for Molecular Life Sciences (BMLS) of Goethe University in Frankfurt, together with colleagues at the Max Planck Institute of Biophysics, has now discovered how yeast cells measure the availability of saturated and unsaturated fatty acids in foodstuffs and adapt their production of membrane lipids to it. This opens up new possibilities to understand the production and distribution of fatty acids and cholesterol in our body cells and make them controllable in future, report the researchers in the latest issue of the “Molecular Cell” journal.
A glance in the supermarket refrigerator shows: Low fat, less fat and no fat are en vogue. Yet fats are essential for cell survival as they form the basic structure for the biological membranes which separate cells from the environment and form functional units inside them. In this way, opposing reactions, such as the formation of energy stores and consumption of fat, can be organised in one and the same cell.
“Membrane lipids have a large number of vital cellular functions. They impact on signal transmission from cell to cell, but also affect intracellular communication,” explains Professor Robert Ernst, whose research group at the BMLS has been on the trail of fats’ hidden functions for years. “Hormone-producing cells are particularly susceptible to perturbed fatty acid metabolism and often have difficulties in regulating their membrane lipid composition. A malfunction of fatty acid regulation can, however, lead to cell death and – depending on the type of cell – trigger diseases such as diabetes.”
First observations that living organisms such as bacteria can actively control their fatty acid production were already made decades ago. Yet until recently researchers puzzled over how higher organisms, for example fungi such as baker’s yeast, measure and regulate the ratio of saturated and unsaturated fatty acids in their membrane lipids. Thanks to funding from the German Research Foundation and the Max Planck Society, the working groups headed by Robert Ernst at Goethe University Frankfurt and Gerhard Hummer at the Max Planck Institute of Biophysics have been able to investigate this fundamentally important question.
In order to describe the mechanism of a membrane sensor which measures the degree of lipid saturation in the yeast cell, the researchers used genetic and biochemical methods and simulated the motions and underlying forces of membrane lipids over a period of a few milliseconds by means of extensive molecular dynamic simulations.
These efforts revealed that the sensing mechanism is based on two cylinder-shaped structures which are positioned next to each other in biological membranes. They both exhibit a rough and a smooth surface respectively and rotate around each other. “It’s like a finger in cookie dough that checks how much butter has been added,” explains Robert Ernst. As saturated fats cannot be accommodated by the rough surface of the helix while unsaturated fats can, the fat sensor’s structure changes depending on the membrane environments. Intriguingly, this conformational change can control the downstream production of unsaturated fatty acids.
“This finding paves the way for many more studies”, predicts Robert Ernst. “With our knowledge of this delicate mechanism in yeast we can now focus on finding new sensors in different organelles and species which monitor and control the production of unsaturated fatty acids and cholesterol in our body.” In view of the far-reaching potential of these findings, an international conference will be staged in the near future. The organisers, including researchers from Frankfurt, expect that many cellular functions of membrane lipids will be revisited from a new perspective and that it will be possible to support hormone-producing cells in a more targeted manner.
Roberto Covino, Stephanie Ballweg, Claudius Stordeur, Jonas B. Michaelis, Kristina Puth, Florian Wernig, Amir Bahrami, Andreas M. Ernst, Gerhard Hummer, and Robert Ernst: A Eukaryotic Sensor for Membrane Lipid Saturation, Molecular Cell (2016), http://dx.doi.org/10.1016/j.molcel.2016.05.015
A video of the dancing fat sensors can be found under:
Further information: Prof. Robert Ernst, Buchmann Institute for Molecular Life Sciences, Riedberg Campus, Tel.: (069) 798-42524,email@example.com.
Exceptional dissertations on intellectual and industrial property law and financial market supervision in the U.S.
Law students Anja Becker and Jenny Gesley have received the 2015 Baker & McKenzie Award for the best dissertations in commercial law at Goethe University in Frankfurt. The two winners received the award during the doctoral awards ceremony at the law department. The Award, which has been given to the authors of exceptional dissertations and professorial theses in the area of commercial law every year since 1988, is given alongside a monetary prize of EUR 6,000.
Anja Becker convinced the jury with her dissertation on "Coordination of proceedings in transnational intellectual and industrial property law disputes" (Verfahrenskoordination bei transnationalen Immaterialgüterrechtsstreitigkeiten). Intellectual and industrial property law is about "intellectual property", which includes patent law, copyright law and trademark law. The awardee examined the question of how parallel, yet related, proceedings can be coordinated, a topic of relevance where intellectual and industrial property law and international private and civil proceedings law intersect. "On the whole, Anja presented a thesis displaying top-notch reasoning on all levels of scientific legal work. This applies to the interpretation of the Brussels Ibis Regulation, the systematization of different case groups from a comparative perspective and last but not least, the prospect of future improvements in the coordination of transnational intellectual and industrial property law disputes", says Prof. Dr. Alexander Peukert, the Chair for Civil Law and Commercial Law at Goethe University and academic supervisor of the award-winning thesis.
Jenny Gesley's thesis "Financial markets supervision in the U.S. National developments and international standards" (Die Aufsicht über die Finanzmärkte in den USA. Nationale Entwicklungen und internationale Vorgaben) conveys a clear and concise impression of the efforts made by U.S. Americans to avert hazards originating from the financial markets with the help of legal measures, says Prof. Dr. Dr. h.c. Helmut Siekmann, academic supervisor of this thesis. He is Deputy Managing Director of the Institute for Monetary and Financial Stability (IMFS) of the House of Finance and holder of the Endowed Chair of Money,Currency and Central Bank Law at Goethe University. He referred to the thesis as an "impressive accomplishment", stating that, "it is in fact partially on a par with a professorial thesis". By consistently pursuing her chosen approach based on historical development, Jenny Gesley had successfully managed to present comprehensive findings for financial markets as a whole.
All dissertations that focus on aspects of commercial law and have been granted "summa cum laude" honors are taken into consideration for the Baker & McKenzie Award each year, as well as professorial theses on aspects of commercial law that were written within an academic year. "By sponsoring the award, we emphasize our close ties with Goethe University and how important the promotion of young legal talent is to our law firm," says Dr. Christian Reichel, member of the management team of Baker & McKenzie Germany and Austria who will hand over the award to the two winners. The law firm has been sponsoring students of Goethe University in the National Scholarship Program from its creation, and Baker & McKenzie attorneys have a long tradition of teaching there.
The crucial step takes place in the dark
FRANKFURT. Birds have a light-dependent compass in their eyes. This compass gives them information about the direction of the Earth's magnetic field. Prof. Roswitha Wiltschko's research groupat Goethe University Frankfurt, together with French colleagues, has elucidated how this compass works at the molecular level.
Birds have two sensory organs for orientation and navigation in the Earth's magnetic field: They use their beak to measure the strength of the magnetic field, while their eyes provide directional information. One type of cone photoreceptors in the birds' eyes is sensitive to UV light and also contains a form of the protein cryptochrome. Previous studies of the Frankfurt researchers suggested that most likely it is this protein that enables birds to detect the magnetic field.
A cyclic reaction involving one light-dependent and one light-independent step takes place in the cryptochrome. Two radical pairs are formed during this cycle, and their unpaired valence electrons react to magnetic fields. The Frankfurt group, working in collaboration with Pierre and Marie Curie University Paris, have now discovered which of these two radical pairs is crucial for navigation in the Earth's magnetic field.
In a behavioural study on robins, the birds were subjected to two experimental conditions: (1) at one-second intervals, the researchers switched off either the light or the Earth's magnetic field while keeping the other stimulus constant; (2) the stimuli alternated in one-second intervals, such that light and magnetic field were not present at the same time. Even in the latter condition the birds could still orient along the Earth's magnetic field lines. The group concludes that the light-independent radical pair is responsible for detecting the magnetic field lines. Light is only required to keep the cycle going.
"This is the first proof that the radical pair generated in darkness is the crucial one for the magnetic compass", says Prof. Roswitha Wiltschko. Since in other organisms cryptochrome is used exclusively for the perception of light, the study indicates that there has been a special evolutionary adaptation in birds.
Information: Prof. Roswitha Wiltschko & Christine Nießner, Institute for Ecology and Diversity, Prof. Wolfgang Wiltschko, Institute for Cell Biology and Neuro Sciences; Phone +49(0)69 798-42119 or +49(0)6032 81206; firstname.lastname@example.org; email@example.com.
Publication: Wiltschko R, Ahmad M, Nießner C, Gehring D, Wiltschko W. 2016 Light-dependent magnetoreception in birds: the crucial step occurs in the dark.J. R. Soc. Interface 20151010.
The scent from pine forests cooled the atmosphere/ Publications in Nature and Science
FRANKFURTAerosol particles generated by human activity counteract the warming of the earth's atmosphere by greenhouse gases. However, this effect might be smaller than first thought, as many particles were already generated from tree emissions in pre-industrial times. This was the finding of a simulation carried out as part of the international CLOUD experiment, in which researchers from the Goethe University played a major role. The results are published in the form of three papers in the renowned journals "Science" and "Nature".
"These results are the most important so far by the CLOUD experiment at CERN", said CLOUD spokesperson Jasper Kirkby, Honorary Professor at the Goethe University. "When the nucleation and growth of pure biogenic aerosol particles is included in climate models, it should sharpen our understanding of the impact of human activities on clouds and climate."
Professor Joachim Curtius from the Institute for Atmospheric and Environmental Sciences at the Goethe University added: "We believe that the newly discovered process will mean that we will have to reassess cloud formation in earlier times, as there must have been more particles present than we had previously assumed. There would therefore be less of a difference between the situation then and now than previously thought."
The CLOUD experiment is looking at how new aerosol particles form in the atmosphere and their effect on climate. As the aerosol particles increase, as is the case due to human activities, more sunlight is reflected and more cloud droplets form, making the clouds brighter. In order to estimate the cooling effect caused by anthropogenic influences, it is necessary to know the quantities of aerosols present in the pre-industrial age. As direct measurement is not an option, the effects are simulated through reliable laboratory tests such as the CLOUD experiment, and then applied to climate modelling.
In pre-industrial times, the organic compounds emitted by trees were a major contributing factor in the formation of aerosols. The researchers examined alpha-pinene, a substance that gives pine forests their characteristic pleasant smell. They are among the most important biogenic emissions. Alpha-pinene is rapidly oxidised on exposure to ozone and the ensuing reaction chains create some extremely low-volatility substances. However, these only occur in very small concentrations of around one molecule per one trillion air molecules.
The CLOUD experiments show that these extremely low-volatility organic compounds are very efficient at forming new particles. This process occurs under atmospheric conditions, even in the absence of sulphuric acid. It had been assumed that sulphuric acid was virtually always involved in particle formation in the atmosphere. The main source of sulphuric acid in the atmosphere is sulphur dioxide, which is generated by the burning of fossil fuels.
Furthermore, the researchers discovered that ions from cosmic rays strongly enhance the production rate of the organic particles - by a factor of 10-100 compared to particle formation without ions, provided the concentrations of the particle-forming gases are low. "Furthermore, our studies show that these low-volatility organic substances also dominate particle growth in unpolluted environments across the entire size range from clusters of just a few molecules all the way up to sizes of 50-100 nm, where the particles are large enough to be able to seed cloud droplets", explained Joachim Curtius. The growth rates accelerate as the particles increase in size, because more and more oxidation products, also those of higher volatility, are able to condense on the expanding particles. This process is described in quantitative terms with a condensation model for the various organic substances.
Why is this knowledge important for our understanding of the climate? This may well be a very important mechanism, because it is so efficient in terms of the formation of organic particles under natural conditions. As soon as the particles have formed, they grow through the condensation of other similar oxygenated organic compounds. The rapid growth of the newly-formed particles means that they lose a smaller percentage through collisions with pre-existing large particles. As a result more particles grow to sizes that have the potential to seed clouds and influence the climate.
Another paper that appears in the same issue of "Science" reports on observations from the observatory on the Jungfraujoch, which detected pure organic nucleation in the free troposphere. This proves the relevance of the CLOUD laboratory experiments for the atmosphere.
Here you can find an image to download.
Caption (fltr): Mario Simon, Martin Heinritzi, Andreas Kürten, Andrea Wagner and Joachim Curtius with the mass spectrometer they developed. This is used to measure the highly oxygenated multifunctional organic molecules and molecule clusters, which are responsible for particle generation and particle growth in the recent CLOUD experiments.
Kirkby, J. et al.: Ion-induced nucleation of pure biogenic particles, in: Nature, doi:10.1038/nature17953
Tröstl, J. et al.: The role of low-volatility organic compounds in initial particle growth in the atmosphere, in: Nature, doi:10.1038/nature18271.
Bianchi, F. et al.: New particle formation in the free troposphere: A question of
chemistry and timing, in: Science, doi 10.1126/science.aad5456, 2016
Information: Prof. Dr. Joachim Curtius, Institute for Atmosphere and Environment, Goethe University, Campus Riedberg, Phone +49(0)69 798-40258, firstname.lastname@example.org.
Five Frankfurt physicists receive the Helmholtz Award with an endowment of Euro 20,000
This year the most important award in the field of metrology, the science of making precise measurements, was awarded to a team of five Frankfurt atomic physicists at Goethe University: Prof. Reinhard Dörner, Associate Prof. Dr. Till Jahnke, Dr. Maksim Kunitzki, Dr. Jörg Voigtsberger and Stefan Zeller. The Helmholtz award includes an endowment of Euro 20,000 and is awarded to European researchers every three years.
The award recipients succeeded in measuring the extremely weak binding energy of helium molecules with a previously unachievable precision. Chemistry teaches us that helium as a noble gas doesn't form bonds. However, this becomes possible under certain circumstances predicted by quantum theory. The study group under Dörner has measured this binding energy indirectly with the COLTRIMS reaction microscope developed at Goethe University. It can be used to measure the location and speed of decaying molecules at the same time with a high level of accuracy, and this data can be used to reconstruct the original configuration. The award winners focused on rare molecules composed of two or three helium atoms.
"It started with the German Research Foundation approving me for a Koselleck project with funding of over 1.25 million in 2009. This is a kind of venture capital, which the DFG uses to support experiments with a long lead time", Prof. Reinhard Dörner from the Institute for Nuclear Physics explains. Dr. Till Jahnke laid the foundation for the equipment, then doctoral candidate Jörg Voigtsberger took over the experiment and achieved initial successes. The next doctoral candidate, Stefan Zeller, was able to make significant improvements to the equipment and to further increase the precision. To do so, he had to direct the largest "photon canon" in Germany, the "Free Electron Laser FLASH" at the DESY research centre in Hamburg, at the extremely weakly bonded helium molecules. In this way he was able to determine the binding energy with a precision of a few nano-electron volts. This means that the binding energy of the helium molecules is one-hundred million times weaker than in a water molecule, for example.
The series of experiments culminated this past year with the dicovery of the so-called Efimov state for a helium molecule made up of three atoms. This comparatively huge molecule predicted 40 years ago by the Russian theorist Vitaly Efimov, can only exist in the tunnel effect established by quantum physics. The postdoc Maksim Kunitzki succeeded in making this measurement with the same equipment.
"All Helmholtz Award winners to date have significantly advanced the art of measuring and many of them are among the most renowned researchers in the field of metrology today", said Dr. Joachim Ullrich, President of the National Metrology Institute of Germany (PTB) and Chairman of the Helmholtz Fund. "We are confident that this will also hold true this time." Researchers at the University of Cambridge are also distinguished with the Helmholtz Award for their method of measuring individual molecules using nano-pores, a proven method in DNA analysis. They have created a method for theoretically detecting any number of different protein molecules in the same measurement. The award will be presented on 22 June 2016 in the conference centre of the National Metrology Institute of Germany (PTB).
The Frankfurt award winners have already received several other rewards for their work: In 2013 Till Jahnke was awarded the most important young scientistaward of the Deutsche Physikalische Gesellschaft, the Gustav Hertz Award. The following year, Reinhard Dörner was distinguished with the renowned Robert Wichard Pohl Award by the Deutsche Physikalische Gesellschaft.In 2015, Maksim Kunitski received an award from the "Frankfurter Förderverein für physikalische Grundlagenforschung".
A picture is available for downloading here: www.uni-frankfurt.de/61204234
Caption: Prof. Reinhard Dörner (left) and Maksim Kunitski in front of the equipment used to complete the outstanding work.
Information: Prof. Reinhard Dörner, Institut für Kernphysik, Max-von-Laue-Str. 1, Tel: (069) 798-47003, email@example.com