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German-American research team deciphers evolution of pathogenic Acinetobacter strains
Hospital-acquired infections (HAIs) are often particularly difficult to treat because the pathogens have developed resistance to common antibiotics. The bacterium Acinetobacter baumannii is particularly dreaded in this respect, and research is seeking new therapeutic approaches to combat it. To look for suitable starting points, an international team led by bioinformaticians at Goethe University Frankfurt has compared thousands of genomes of pathogenic and harmless Acinetobacter strains. This has delivered clues about which properties might have made A. baumannii a successful pathogen – and how it might possibly be combated.
FRANKFURT/ST.
LOUIS. Each year, over 670,000 people in Europe fall ill
through pathogenic bacteria that exhibit antibiotic resistance, and 33,000 die
of the diseases they cause. Especially feared are pathogens that are resistant
to several antibiotics at the same time. Among them is the bacterium Acinetobacter baumannii, which is today
dreaded above all as a “hospital superbug": up to five percent of all
hospital-acquired bacterial infections are caused by this germ alone.
A. baumannii is right at the top of a list of candidates for
which, according to the World Health Organization (WHO), new therapies must be
developed. This is because the pathogen – due to a flexible genome – easily
acquires new antibiotic resistance. At the same time, infections are not only occurring
more and more outside the hospital environment but also leading to increasingly
severe progression. However, a prerequisite for the
development of new therapeutic approaches is that we understand which
properties make A. baumannii and its human pathogenic relatives, grouped
in what is known as the Acinetobacter calcoaceticus-baumannii (ACB) complex,
a pathogen.
A team led by bioinformatician Professor
Ingo Ebersberger from Goethe University Frankfurt/ LOEWE Centre for
Translational Biodiversity Genomics (LOEWE-TBG) has now reached a milestone in
this understanding. The team is composed of members of Research Unit 2251 of
the German Research Foundation and other national and international partners,
among them scientists of Washington University School of Medicine, St Louis,
USA.
For their analysis, the team made use of
the fact that a large proportion of the members of the Acinetobacter
genus are harmless environmental bacteria that live in water or on plants or
animals. Thousands of complete genome sequences both of these as well as of
pathogenic Acinetobacter strains are stored in publicly accessible
databases.
By comparing these genomes, the
researchers were able to systematically filter out differences between the
pathogenic and the harmless bacteria. Because the incidence of individual genes
was not particularly conclusive, Ebersberger and his colleagues concentrated on
gene clusters, that is, groups of neighbouring genes that have remained stable
during evolution and might form a functional unit. “Of these evolutionarily
stable gene clusters, we identified 150 that are present in pathogenic Acinetobacter
strains and rare or absent in their non-pathogenic relatives," says
Ebersberger, summing up. “It is highly probable that these gene clusters benefit
the pathogens' survival in the human host."
Among the most important properties of
pathogens is their ability to form protective biofilms and to efficiently
absorb micronutrients such as iron and zinc. And indeed, the researchers
discovered that the uptake systems in the ACB group were a reinforcement of the
existing and evolutionary older uptake mechanism.
Particularly exciting is the fact that the
pathogens have evidently tapped a special source of energy: they can break down
the carbohydrate kynurenine produced by humans, which as a messenger substance regulates
the innate immune system. The bacteria apparently kill two birds with one stone
in this way. On the one hand, breaking down kynurenine supplies them with
energy, and on the other hand, they could possibly use it to deregulate the
host's immune response.
Ebersberger is convinced: “Our work is a
milestone in understanding what's different about pathogenic Acinetobacter
baumannii. Our data are of such a high resolution that we can even look at
the situation in individual strains. This knowledge can now be used to develop
specific therapies against which, with all probability, resistance does not yet
exist."
Publication:
Bardya Djahanschiri, Gisela Di Venanzio,
Jesus S. Distel, Jennifer Breisch, Marius Alfred Dieckmann, Alexander Goesmann,
Beate Averhoff, Stephan Göttig, Gottfried Wilharm, Mario F. Feldman, Ingo
Ebersberger: Evolutionarily stable gene
clusters shed light on the common grounds of pathogenicity in the Acinetobacter
calcoaceticus-baumannii complex. PLOS Genetics (2022) DOI:
10.1371/journal.pgen.1010020 https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010020
Picture
download:
Acinetobacter baumannii
https://commons.wikimedia.org/wiki/File:Acinetobacter_baumannii.JPG
Scanning electron micrograph of a cluster
of Gram-negative, immobile bacteria of the Acinetobacter
baumannii species. Photo:
Janice Carr
Further
information:
Professor Ingo Ebersberger
Institute of Cell Biology and Neuroscience
Goethe University Frankfurt
Tel.: +49 69 798 42112
ebersberger@bio.uni-frankfurt.de
Homepage: http://www.bio.uni-frankfurt.de/43045195/ak-ebersberger
Goethe University Frankfurt’s Science Magazine, Forschung Frankfurt, on the topic of motion has now been published in English – New Priority Programme focuses on facial and manual gestures
Communication consists not only of spoken words and phrases. We also convey important information by gesturing with our arms, hands and face. Visual communication, a field so far scarcely studied by theoretical linguistics, is the focus of a new Priority Programme of the German Research Foundation coordinated by Goethe University Frankfurt. Read more in the current issue of “Forschung Frankfurt" entitled “In motion".
FRANKFURT. How
gestures and facial expressions can underline, supplement and modify the
meaning of words and phrases is something that several disciplines at Goethe
University Frankfurt are exploring. Linguistics professor Cornelia Ebert is
interested in how the contribution of the meaning of gestures can be modelled.
Until recently, visual contributions to meaning were not dealt with in formal
linguistics, but instead first and foremost in communication sciences as well
as in rhetoric, semiotics and psychology.
Together with Professor Markus Steinbach,
sign language researcher at the University of Göttingen, Ebert has successfully
applied for a Priority Programme of the German Research Foundation and is
responsible for its coordination. The objective is to bring together existing
findings from various disciplines and link them with linguistics. You can read
about the research questions that the programme will address in the latest
issue of Forschung Frankfurt, the Science Magazine of Goethe University
Frankfurt, which is dedicated to the topic of motion.
In other articles, scientists from Goethe
University Frankfurt report on their research projects related to various
aspects of motion, for example how they teach computers to recognise different
movements such as “cutting" or “waving", how ADHD can affect adults too or how
two movements in quantum physics are superimposed, each of which only occurs
with a certain probability. Other articles explore, for example, how
smartphones, which are almost ubiquitous, are changing film as a medium or how
sports clubs can foster the integration of immigrants.
Journalists can order the current
English-language issue of Forschung Frankfurt (2/2021) free of charge from:
ott@pvw.uni-frankfurt.de
All articles are available online at
www.forschung-frankfurt.de (then go to EN)
or https://tinygu.de/ENForschungFrankfurt
Editor: Dr Markus Bernards, Science Editor, PR & Communication Office, Tel: -49 (0) 69 798-12498, Fax: +49 (0) 69 798-763 12531, bernards@em.uni-frankfurt.de
Bi-directional binding and release of hydrogen in bioreactor
A team of microbiologists from Goethe University Frankfurt has succeeded in using bacteria for the controlled storage and release of hydrogen. This is an important step in the search for carbon-neutral energy sources in the interest of climate protection. The corresponding paper has now been published in the renowned scientific journal “Joule".
FRANKFURT. The
fight against climate change is making the search for carbon-neutral energy
sources increasingly urgent. Green hydrogen, which is produced from water with
the help of renewable energies such as wind or solar power, is one of the
solutions on which hopes are pinned. However, transporting and storing the
highly explosive gas is difficult, and researchers worldwide are looking for
chemical and biological solutions. A team of microbiologists from Goethe
University Frankfurt has found an enzyme in bacteria that live in the absence
of air and bind hydrogen directly to CO2, in this way producing
formic acid. The process is completely reversible – a basic requirement for
hydrogen storage. These acetogenic bacteria, which are found, for example, in
the deep sea, feed on carbon dioxide, which they metabolise to formic acid with
the aid of hydrogen. Normally, however, this formic acid is just an
intermediate product of their metabolism and further digested into acetic acid
and ethanol. But the team led by Professor Volker Müller, head of the
Department of Molecular Microbiology and Bioenergetics, has adapted the
bacteria in such a way that it is possible not only to stop this process at the
formic acid stage but also to reverse it. The basic principle has already been
patented since 2013.
“The measured rates of CO2
reduction to formic acid and back are the highest ever measured and many times
greater than with other biological or chemical catalysts; in addition, and unlike
chemical catalysts, the bacteria do not require rare metals or extreme
conditions for the reaction, such as high temperatures and high pressures, but instead
do the job at 30 °C and normal pressure," reports Müller. The group now has a
new success to report: the development of a biobattery for hydrogen storage with
the help of the same bacteria.
For municipal or domestic hydrogen
storage, a system is desirable where the bacteria first store hydrogen and then
release it again in one and the same bioreactor and as stably as possible over
a long period of time. Fabian Schwarz, who wrote his doctoral thesis on this
topic at Professor Müller's laboratory, has succeeded in developing such a
bioreactor. He fed the bacteria hydrogen for eight hours and then put them on a
hydrogen diet during a 16-hour phase overnight. The bacteria then released all the
hydrogen again. It was possible to eliminate the unwanted formation of acetic
acid with the help of genetic engineering processes. “The system ran extremely
stably for at least two weeks," explains Fabian Schwarz, who is pleased that
this work has been accepted for publication in “Joule", a prestigious journal
for chemical and physical process engineering. “That biologists publish in this
important journal is somewhat unusual," says Schwarz.
Volker Müller had already studied the
properties of these special bacteria in his doctoral thesis – and spent many years
conducting fundamental research on them. “I was interested in how these first
organisms organised their life processes and how they managed to grow in the absence
of air with simple gases such as hydrogen and carbon dioxide," he explains. As
a result of climate change, his research has acquired a new, application-oriented
dimension. Surprisingly for many engineers, biology can produce by all means practicable
solutions, he says.
Publication:
Fabian M. Schwarz, Florian Oswald, Jimyung Moon, Volker Müller: Biological hydrogen
storage and release through multiple cycles of bi-directional hydrogenation of
CO2 to formic acid in a single process unit. Joule (2022) https://doi.org/10.1016/j.joule.2022.04.020
Picture
download: https://www.uni-frankfurt.de/119545783
Caption:
Model of a potential
bacterial hydrogen storage system: during the day, electricity is generated
with the help of a photovoltaic unit, which then powers the hydrolysis of
water. The bacteria bind the hydrogen produced in this way to CO2,
resulting in the formation of formic acid. This reaction is fully reversible,
and the direction of the reaction is steered solely by the concentration of the
starting materials and end products. During the night, the hydrogen
concentration in the bioreactor decreases and the bacteria begin to release the
hydrogen from the formic acid again. This hydrogen can then be used as an
energy source.
Further
information
Professor Volker Müller
Department of Molecular Microbiology & Bioenergetics
Institute for Molecular Biosciences
Goethe University Frankfurt
Tel.:
+49 (0)69 798-29507
vmueller@bio.uni-frankfurt.de
Editor: Dr. Anke Sauter, Science Editor, PR & Communication Office, Tel. +49 69 798-13066, Fax + 49 69 798-763-12531, sauter@pvw.uni-frankfurt.de
Team from Goethe University contributes to article in “Nature”
Atmospheric researchers from the international CLOUD consortium have discovered a mechanism that allows nuclei for ice clouds to form and rapidly grow in the upper troposphere. The discovery is based on cloud chamber experiments to which a team from Goethe University contributed highly specialised measurements. Although the conditions for nucleus formation are only fulfilled in the Asian monsoon region, the mechanism is expected to have an impact on ice cloud formationacross large parts of the Northern Hemisphere (Nature DOI 10.1038/s41586-022-04605-4)
FRANKFURT. The
Asian monsoon transports enormous amounts of air from atmospheric layers close
to Earth's surface to a height of around 15 kilometres. Like in a gigantic
elevator, human-induced pollutants also end up in the upper troposphere in this
way. A research team from the CLOUD consortium (Cosmics Leaving Outdoor
Droplets), including atmospheric researchers from Goethe University in
Frankfurt, have reproduced the conditions prevailing there, among them cosmic
radiation, in their experimental chamber at the CERN particle accelerator
centre in Geneva.
In the process, they identified that up to
100 times more aerosol particles form from ammonia, nitric acid and sulphuric
acid than when only two of these substances are present. These particles are
then available on the one hand as condensation nuclei for liquid water droplets
in clouds and on the other hand as solid seeds for pure ice clouds, so-called
cirrus clouds. The research team also observed that ice clouds with the
three-component particles already form at lower water vapour supersaturation
than anticipated. This means that the ice clouds already develop under
conditions that atmospheric researchers worldwide had so far assumed did not
lead to the formation of cirrus clouds. With model calculations from around the
globe, the CLOUD research team was also able to show that the cloud nuclei can spread
across large parts of the Northern Hemisphere within just a few days.
“The experiment in the cloud chamber was a
reaction to the results of field experimentsover Asia. These measurements showed
that ammonia is present there in the upper troposphere during the monsoon,"
explains Professor Joachim Curtius from Goethe University. “Previously, we had
always assumed that ammonia, due to its water solubility, was rinsed out of the
rising air masses before it reached the upper troposphere." As the CLOUD
researchers' experiment now corroborates, ammonia is an essential ingredient
for more cloud formation. Ammonia emissions in Asia come predominantly from
agriculture.
The international CLOUD research collaboration
(Cosmics Leaving Outdoor Droplets) is made up of teams from 21 research
institutions. In the experiment of which the research team is now presenting the
results in the current issue of “Nature", the researchers led by Curtius were
responsible for the mass spectrometric measurement of the sulphuric acid
concentration. This concentration changed over the course of the experiment,
but was still always very low, like in the upper troposphere: for a single
sulphuric acid molecule there are over a trillion other gas molecules. “Apart
from the very best measuring equipment, such measurements require highly
specialised expertise. That is why you need teams with complementary skills to
conduct such an experiment," explains Curtius, who is a member of the CLOUD
steering committee and was coordinator of the EU project CLOUD-MOTION
successfully completed just recently. Like in the atmosphere, sulphuric acid
forms in the CLOUD chamber from sulphur dioxide and hydroxyl radicals.
Clouds are an important and at the same
time still insufficiently understood element of global climate. Depending on
whether they float high up or low down, their water or ice content, how thick
they are or over which region of the globe they form, it gets warmer or colder beneath
them. To improve the precision of climate models, researchers worldwide require
exact knowledge of all the processes surrounding clouds as a climate factor.
The CLOUD research team's findings are helping them a long way towards increasingly
reliable climate predictions.
Publication:
Mingyi Wang et al., Synergistic HNO3 H2SO4 NH3
upper tropospheric particle formation. Nature https://www.nature.com/articles/s41586-022-04605-4, DOI 10.1038/s41586-022-04605-4
Picture
download:
https://www.uni-frankfurt.de/119325153
Caption:
Air pollutants form the condensation
nuclei for ice clouds or cirrus clouds (here: Cirrus spissatus). When ammonia,
nitric acid and sulfuric acid are present together, they form such condensation
nuclei particularly effectively. Credit: Joachim Curtius, Goethe-University
Frankfurt
Further
information:
Professor Joachim Curtius
Institute for Atmospheric and Environmental Sciences
Goethe University, Frankfurt, Germany
Phone +49 (0)69 798-40258
curtius@iau.uni-frankfurt.de
A sociological study at Goethe University Frankfurt examines the attitudes of migrants in Europe
The police – your friend and helper? For people immigrating into Europe from another country, this is not always the case. A study at Goethe University shows how the relationship to state power develops among different immigrant groups.
FRANKFURT. The murder of African-American George Floyd in May 2020 led to worldwide protests against police violence. Not least because of these developments, in Europe, too, the relationship between the police and ethnic minorities has been a hotly debated topic in the recent past.
A study by Christian Czymara of Goethe University Frankfurt and Jeffrey Mitchell of Umeå University (Sweden), which has just been published, also focuses on the trust placed in the police by immigrants in Europe. The two social scientists have analysed the data of almost 20,000 immigrants from 22 European countries in the period from 2006 to 2019. These data, which originate from the European Social Survey, show that trust in the police is indeed on average higher among immigrants than among the native population. However, the longer people live in the destination country, the more trust tends to erode.
The European Social Survey asks interviewees about their trust in various institutions, which they rank on a scale of 0 to 10. Over half the interviewees originally come from other European countries, 12 percent from Africa, 25 percent from Asia.
The authors have two explanations for the fact that trust decreases with the length of stay: first, the memory of the country of origin, and of the situation there, fades. The contrast between the country of origin and the country of destination is particularly significant for people who have immigrated from countries with a lower level of rule of law to a country that is very advanced in that respect. The second explanation is that these people often experience discrimination in their new surroundings, especially those belonging to an ethnic minority there. This is indicated by the fact that the effect of people's experiences of discrimination is stronger for those who have been in the destination country for longer than for those who have recently arrived. Moreover, comparisons between European countries clearly demonstrate that trust is on average lower where there are more police – for example, in Cyprus, Croatia and Greece. The authors conclude that the size of a police force alone can hardly boost trust in the police, but instead experiences of discrimination must be reduced. Accordingly, efforts in this area would help to maintain the high level of trust in the police among new immigrants and to restore the trust of those who have lived in their host country for a long time.
Publication: Czymara & Mitchell (2022). All Cops are Trusted? How Context and Time Shape Immigrants' Trust in the Police in Europe. Ethnic and Racial Studies. https://www.tandfonline.com/doi/full/10.1080/01419870.2022.2060711
Further information
Dr Christian Czymara
Research Associate
Institute of Sociology
Goethe University Frankfurt
Tel.: +49 69 798 36708
czymara@soz.uni-frankfurt.de
https://www.fb03.uni-frankfurt.de/74691200/czymara