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Jörg |
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Eric |
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Merck-Stiftungsprofessur Molecular Biotechnology |
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Helge |
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Volker |
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Beate |
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Molecular Cell Biology of Plants |
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Enrico |
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mRNA-based gene regulation |
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Andreas |
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Plant Cell Physiology |
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Claudia |
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Physiology and Genetics of Lower Eukaryotes |
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Prof. |
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Eckhard |
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RNA Regulation in Higher Eukaryotes |
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Prof. |
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Michaela |
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RNA Structural Biology |
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Prof. |
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Jens |
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Beate |
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Claudia |
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Helge |
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Eckhard |
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Prof. | Eric | Helfrich | Natural Product Genomics | |||
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Volker |
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Michaela |
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Enrico |
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Andreas |
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Jörg |
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Jens |
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The talks starts at 17:15
Campus Rieberg, Biocenter, Section of the Building 260 Room 3.13
Jan Fíla, Institute of Experimental Botany of the CASBiologicum, Prague, Czech Republic
Thursday, December 7th - 17:00 - Biocenter, Hörsaal B2
The male gametophyte of flowering plants (Angiospermae) represents a unique model on which various processes can be studied at the level of a single cell, for example the study of ion gradients, vesicle transport, cytoskeleton dynamics, but also translational regulation and protein phosphorylation. The last process has been studied in our experiments, which have led to the identification of a number of phosphorylated proteins with exact position of their phosphorylation sites. From the tobacco pollen phosphoproteome, several protein candidates were selected for subsequent detailed functional analyses.
The heterodimeric nascent polypeptide-associated complex (NAC) represents one of these studied candidates. In Arabidopsis thaliana genome, there are five genes encoding the NACα-subunit, and two genes encoding the NACβ-subunit. The double homozygous mutants of both NACβ genes were acquired by a conventional cross of two available T-DNA insertion lines. These double homozygous mutants showed several phenotypic traits different from the Columbia-0 wild type plants, such as delayed development, abnormal number of flower organs, and abnormally short siliques, which carried a lower number of seeds. Both NACβ genes were localized to nuclei and cytoplasm and their promoters were active in many organs (leaves, cauline leaves, flowers, pollen grains, and siliques together with seeds). Since flowers were the most affected organs by nacβ mutation, the transcriptome of flower buds was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NACβ subunits in stress responses, male gametophyte development, and photosynthesis.
Acknowledgment: The authors gratefully acknowledge the financial support from Czech Science Foundation (22-29717S, 23-07000S), and Mobility Plus Program with DAAD (DAAD-23-06).
Invited by Dr. Fragkostefanakis
Prof. Yvonne Stahl,
Institut für Molekulare Biowissenschaften, Fachbereich Biowissenschaften, Goethe
Universität Frankfurt
Tuesday, January 23rd 2024, 17:00, Hörsaal 3, Otto-Stern-Zentrum
The root
system of higher plants originates from the activity of a root meristem
comprising a group of highly specialized and long-lasting stem cells.
Maintenance and homeostasis of the stem cell niche (SCN) in the root is
essential for plant growth and development and is controlled by feedback
signaling from differentiated cells involving intricate gene regulatory
networks. Although some plant transcription factors (TFs) are known as
important regulators of root SCN maintenance, much of the necessary tight but
also dynamic regulation of the transition from stem cell fate to
differentiation still remains largely elusive.
We found
that key TFs in root SCN regulation contain intrinsically disordered regions
and prion-like domains (PrDs) which are necessary for complex formation with
other TFs and their dynamic subcellular localization to nuclear bodies (NBs).
Furthermore, we observed that the recruitment to these NBs is important for
distal root stem cell homeostasis.
We propose
that the observed partitioning of TF complexes to NBs, possibly by
liquid-liquid phase separation, is important for the determination of distal
stem cell fate. These dynamic translocations could act as another, dynamic
layer of regulation, ensuring stem cell homeostasis, in response to
differential internal and external cues.
Jan Fíla, Institute of Experimental Botany of the CASBiologicum, Prague, Czech Republic
Thursday, December 7th - 17:00 - Biocenter, Hörsaal B2
The male gametophyte of flowering plants (Angiospermae) represents a unique model on which various processes can be studied at the level of a single cell, for example the study of ion gradients, vesicle transport, cytoskeleton dynamics, but also translational regulation and protein phosphorylation. The last process has been studied in our experiments, which have led to the identification of a number of phosphorylated proteins with exact position of their phosphorylation sites. From the tobacco pollen phosphoproteome, several protein candidates were selected for subsequent detailed functional analyses.
The heterodimeric nascent polypeptide-associated complex (NAC) represents one of these studied candidates. In Arabidopsis thaliana genome, there are five genes encoding the NACα-subunit, and two genes encoding the NACβ-subunit. The double homozygous mutants of both NACβ genes were acquired by a conventional cross of two available T-DNA insertion lines. These double homozygous mutants showed several phenotypic traits different from the Columbia-0 wild type plants, such as delayed development, abnormal number of flower organs, and abnormally short siliques, which carried a lower number of seeds. Both NACβ genes were localized to nuclei and cytoplasm and their promoters were active in many organs (leaves, cauline leaves, flowers, pollen grains, and siliques together with seeds). Since flowers were the most affected organs by nacβ mutation, the transcriptome of flower buds was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NACβ subunits in stress responses, male gametophyte development, and photosynthesis.
Acknowledgment: The authors gratefully acknowledge the financial support from Czech Science Foundation (22-29717S, 23-07000S), and Mobility Plus Program with DAAD (DAAD-23-06).
Invited by Dr. Fragkostefanakis
Prof. Yvonne Stahl,
Institut für Molekulare Biowissenschaften, Fachbereich Biowissenschaften, Goethe
Universität Frankfurt
Tuesday, January 23rd 2024, 17:00, Hörsaal 3, Otto-Stern-Zentrum
The root
system of higher plants originates from the activity of a root meristem
comprising a group of highly specialized and long-lasting stem cells.
Maintenance and homeostasis of the stem cell niche (SCN) in the root is
essential for plant growth and development and is controlled by feedback
signaling from differentiated cells involving intricate gene regulatory
networks. Although some plant transcription factors (TFs) are known as
important regulators of root SCN maintenance, much of the necessary tight but
also dynamic regulation of the transition from stem cell fate to
differentiation still remains largely elusive.
We found
that key TFs in root SCN regulation contain intrinsically disordered regions
and prion-like domains (PrDs) which are necessary for complex formation with
other TFs and their dynamic subcellular localization to nuclear bodies (NBs).
Furthermore, we observed that the recruitment to these NBs is important for
distal root stem cell homeostasis.
We propose
that the observed partitioning of TF complexes to NBs, possibly by
liquid-liquid phase separation, is important for the determination of distal
stem cell fate. These dynamic translocations could act as another, dynamic
layer of regulation, ensuring stem cell homeostasis, in response to
differential internal and external cues.
Institute for Moelcular Bio Science
Campus Riedberg
Biocentre
Building N210-207
Post office box 6
Max-von-Laue-Str. 9
D-60438 Frankfurt
T +49 69 798-29603
F +49 69 798-29600
E info-mbw@bio.uni-frankfurt.de
Managing Director:
Prof. Dr. Michaela Müller-McNicoll
Assis. Managing Director:
Prof. Dr. Claudia Büchel
Further information: eMail
Dr. Markus Fauth
Tel: 069 798 29603
Dr. Matthias Rose
Tel: 069 798 29529