Press releases – March 2022

 

Mar 21 2022
14:48

Fundamental research for novel approaches for the control of Trypanosoma parasites

How the Chagas pathogen changes the intestinal microbiota of predatory bugs 

In Central and South America, predatory blood-sucking bugs transmit the causative agent of the widely prevalent Chagas disease. As the disease can induce severe symptoms and to date there is no vaccine against the Trypanosoma parasites, the main approach at present is to control the bug using insecticides. A German-Brazilian research team has now studied how trypanosomes change the bug's intestinal microbiota. The long-term goal: to change the bacterial community in the predatory bug's intestine in such a way that it can defend itself against the trypanosomes.

FRANKFURT. According to estimates by the World Health Organization (WHO), between six and seven million people worldwide, predominantly in Central and South America, are infected with the Trypanosoma cruzi species of trypanosome. This single-celled (protozoan) parasite causes Chagas disease (American trypanosomiasis), which in the acute phase is inconspicuous: only in every third case does the infected person develop any symptoms at all, which can then be unspecific, such as fever, hives and swollen lymph nodes. However, the parasites remain in the body, and many years later chronic Chagas disease can become life-threatening, with pathological enlargement of the heart and progressive paralysis of the gastrointestinal tract. 

There is no vaccine against the pathogen and treating the disease in the advanced stage is difficult. That is why the focus in Latin America is rather on controlling the bug that transmits Chagas trypanosomes: the predatory blood-sucking bug of the insect subfamily Triatominae. It ingests the trypanosomes during the sting, which then colonize its intestine. Through its faeces that it mostly deposited next to the bite, the bug excretes the pathogen, which is often rubbed into the wound when scratching the extremely itchy bite.

Although the number of new infections has dropped in various regions where insecticides are sprayed on a wide scale, problems are emerging: over the last decade, resistance to common insecticides by several species of predatory bugs has been increasingly observed. These insecticides also have a negative impact on the environment and the local population.

Researchers worldwide are making intense efforts to find alternative methods to help control Trypanosoma cruzi. One possibility might be to modify bacteria in the predatory bug's intestine in such a way that they eliminate the Chagas trypanosomes or inhibit their development.

In collaboration with scientists at the Instituto René Rachou in Belo Horizonte, Brazil, parasitologists and infection biologists Fanny Eberhard and Professor Sven Klimpel from Goethe University, the Senckenberg – Leibniz Institution for Biodiversity and Earth System Research (SGN) and the LOEWE Centre for Translational Biodiversity Genomics have now investigated how Chagas trypanosomes change the bacterial community in the predatory bug's intestine. To do so, they used genome analysis, which allowed them to compare the composition of the bacterial community in the bug's intestine, the microbiome, before and after infection with the pathogen (metagenomic shotgun sequencing).

The result: after the infection, the range of bacterial strains in the bug's intestine significantly decreased. Certain strains, including the potentially pathogenic bacterium Enterococcus faecalis, profited from the parasites' presence. Moreover, the researchers succeeded in identifying four bacterial species that probably take on functions important for the bug, such as the synthesis of B vitamins.

Fanny Eberhard explains: “Vitamin B is one of the nutrients that blood-sucking insects do not obtain through their blood meals. Bacteria that produce vitamin B are therefore very important for the bug, are found in practically all individuals and stay in the predatory bug's intestine even across generations. Hence, such bacteria are potentially suitable recipients for genes that produce defensive substances against Chagas trypanosomes."

Professor Sven Klimpel elaborates: “Ultimately, our goal is for the predatory bug to defend itself against Chagas trypanosomes and, in this way, to prevent infection in humans. However, before we can produce bacteria with such properties and then release predatory bugs containing them, we need to understand better how the ecology of the bug's intestine is structured and how the extensive interactions between host, pathogen and microbiome function. Our work is delivering an essential contribution to this."

Publication: Fanny E. Eberhard, Sven Klimpel, Alessandra A. Guarneri, Nicholas J. Tobias. Exposure to Trypanosoma parasites induces changes in the microbiome of the Chagas disease vector Rhodnius prolixus. Microbiome (2022) 10:45. https://doi.org/10.1186/s40168-022-01240-z

Picture download: https://www.uni-frankfurt.de/116081371

Captions:
Rhodnius prolixus_1000px.jpg
The predatory bug Rhodnius prolixus is one of the main vectors of Chagas disease in the north of South America and in Central America. Photo: Dr Erwin Huebner, University of Manitoba, Winnipeg, Canada/ Wikimedia Commons

Rhodnius prolixus_Life_cycle.jpg
Example of the hemimetabolic life cycle of the predatory triatomine bug Rhodnius prolixus. Shown are the adult vector, freshly laid, milky-white eggs, mature, reddish eggs and five nymphs. Red arrows mark a blood meal for the moulting process and egg production. Pictured in the middle are frequent hosts, such as dogs, opossums and humans. Graphics: Fanny E. Eberhard

Further information:
Professor Sven Klimpel
Institute of Ecology, Diversity and Evolution, Goethe University
Senckenberg – Leibniz Institution for Biodiversity and Earth System Research (SGN)
LOEWE Centre for Translational Biodiversity Genomics
Tel. +49 (0)69 798-42249
Klimpel@bio.uni-frankfurt.de
https://www.bio.uni-frankfurt.de/43925886/Abt__Klimpel


Editor: Dr Markus Bernards, Science Editor, PR & Communication Office, Tel: -49 (0) 69 798-12498, Fax: +49 (0) 69 798-763 12531, bernards@em.uni-frankfurt.de 

 

Mar 3 2022
11:42

POLY research group offers fellowships for researchers forced to leave Ukraine

Short-term funding for Ukrainian historians

The POLY research group on premodern Christianities at Goethe University is offering five fellowships to Ukrainian academics specialised in medieval or early modern history.

FRANKFURT. The Russian attack on Ukraine is endangering the lives and work of many researchers. To help some of them to continue their research outside Ukraine, the “Polycentricity and Plurality of Premodern Christianities” (POLY) research group, a Centre for Advanced Studies in Humanities funded by the German Research Foundation, is offering five fellowships. These are intended for scholars with a doctoral degree who are dealing with medieval or early modern history and focus especially on religious diversity.

“With this initiative, we at POLY want to help colleagues from Ukraine forced to flee to safety and to give a stronger voice to Ukrainian science and research,” says Professor Birgit Emich, chair of the POLY fellowship programme, summing up the research group’s motivation. For Emich, who teaches early modern history at Goethe University, the fellowships also offer great opportunities for research in Frankfurt: “With the help of these visiting scholars, we aim to develop further partnerships in this region, which is so rich for the study of religious diversity.”

The fellowships are endowed with €3,000 per month and initially limited to four months. During the funding period, the visiting Ukrainian scholars will not only be integrated in work within POLY but also profit from other research infrastructure at Goethe University, notably, the research alliance “Dynamics of Religion”, co-chaired by Emich and Christian Wiese, theologian and professor for Jewish studies.

Applications for fellowships are now being accepted. They are conditional on a completed doctoral degree and an academic focus on religious plurality in the medieval or early modern period.

Further information
Professor Birgit Emich
Institute of History
Chair of Early Modern History
Goethe University
Tel.: +49 (0) 69 798-32594
Email: emich@em.uni-frankfurt.de
https://www.geschichte.uni-frankfurt.de/92594738/Polycentricity_and_Plurality_of_Premodern_Christianities__POLY


Editor: Dr. Anke Sauter, Science Editor, PR & Communication Office, Tel. +49 69 798-13066, Fax + 49 69 798-763-12531, sauter@pvw.uni-frankfurt.de